Related ArticlesProfilin is a marker of severity in allergic respiratory diseases. Allergy. 2019 Dec 05;: Authors: Ruiz-Hornillos J, López-Matas MA, Berges Jimeno P, Henríquez A, Blanco S, Seoane-Rodríguez M, Mahíllo I, Carnés J Abstract BACKGROUND: The capacity of profilin to induce allergic symptoms in patients with respiratory allergy has been questioned. In this sense, the aim of this study was to investigate the correlation between profilin exposure and induction of symptoms in a prospective case-control study. METHODS: The concentration of profilin as well as pollen levels in the air were measured. A diary score of symptoms was collected from allergic patients. Seventy-nine individuals were included in the study; 51 cases and 28 controls were positive or negative to profilin, respectively. Conjunctival and bronchial provocation tests were performed with purified profilin (Pho d 2) in a subgroup of cases and controls. RESULTS: Profilin was detected in the environment on 133 days (maximum peak of 0.56 ng/m3 ). A positive correlation between profilin and pollen count of Olea and Poaceae was observed (ρ=0.24; p<0.001). Intensity of total, nasal and ocular symptoms was statistically higher in cases than in controls (p<0.001). The risk of suffering symptoms, measured by the percentage of patients who presented any of the symptoms each day, was also higher in cases than in controls. The provocation test was positive in 95% of bronchial and 90% of conjunctival challenges in cases, and negative in all controls. CONCLUSIONS: Profilin was detected in the environment and had the ability to induce a specific allergen response. Patients sensitised to this panallergen showed more symptoms and were more likely to have symptoms. Therefore, sensitisation to profilin seems to be a marker of severity in patients with rhinoconjunctivitis and asthma mediated by pollen.PMID: 31804710 [PubMed - as supplied by publisher]

Related ArticlesSubphenotypes of NSAID-exacerbated respiratory disease identified by latent class analysis. Allergy. 2019 Dec 05;: Authors: Celejewska-Wójcik N, Wójcik K, Ignacak-Popiel M, Ćmiel A, Tyrak K, Gielicz A, Kania A, Nastałek P, Sanak M, Mastalerz L Abstract BACKGROUND: Induced sputum (IS) allows to measure mediators of asthmatic inflammation in bronchial secretions. NSAID-exacerbated respiratory disease (NERD) is recognized as a distinct asthma phenotype, usually with a severe course, eosinophilic airway inflammation and increased production of proinflammatory eicosanoids. A more insightful analysis of NERD patients has shown this phenotype to be nonhomogeneous. OBJECTIVE: We aimed to identify possible subphenotypes in a cohort of NERD patients with the means of latent class analysis (LCA). METHODS: 95 asthma patients with aspirin hypersensitivity underwent sputum induction. High-performance liquid chromatography or gas chromatography coupled with mass spectrometry were used to profile eicosanoids in induced sputum supernatant (ISS). 16 variables covering clinical characteristics, IS inflammatory cells and eicosanoids were considered in the LCA. RESULTS: Three classes (subphenotypes) were distinguished within the NERD cohort. Class 1 subjects had mild to moderate asthma, an almost equal distribution of inflammatory cell patterns, the lowest concentrations of eicosanoids and logLTE4 /logPGE2 ratio. Class 2 represented severe asthma with impaired lung function despite high doses of steroids. High sputum eosinophilia was in line with higher proinflammatory LTE4 in ISS and the highest logLTE4 /logPGE2 ratio. Class 3 subjects had mild to moderate asthma and were also characterized by eosinophilic airway inflammation, yet increased production of pro (LTE4 , PGD2 and 11-dehydro-TBX2 ) was balanced by anti-inflammatory PGE2 . The value of logLTE4 /logPGE2 was between values calculated for class 1 and 3, similarly to disease control and severity. CONCLUSIONS: LCA revealed three distinct NERD subphenotypes. Our results support a more complex pathobiology of aspirin hypersensitivity. Considering NERD heterogeneity, the relationship between inflammatory pathways and clinical manifestations of asthma may lead to more individualized treatment in difficult to treat patients in the future.PMID: 31803947 [PubMed - as supplied by publisher]

Related ArticlesBasophil activation testing, IgG and IgG4 in the diagnosis of dog allergy in children with and without a dog at home. Allergy. 2019 Dec 04;: Authors: Käck U, Asarnoj A, Binnmyr J, Grönlund H, Wallén C, Lilja G, van Hage M, Nopp A, Konradsen JR PMID: 31802499 [PubMed - as supplied by publisher]